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ANALYSIS OF REAL-TIME PCR DATA 1. THE 22DDCT METHOD or X N 3 (1 1 E)DCT 5 K, [6] Derivation of the 22DDCT Method The equation that describes the exponential amplifi- where X N is equal to the normalized amount of target cation of PCR is (X0/R 0) and DC T is equal to the difference in threshold cycles for target and reference (CT,X 2 C T,R.

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Davidson scores 37, hits game-winner for PAC

Rock–paper–scissors (also known as scissors–rock–paper or other variants) is a hand game usually played between two people, in which each player simultaneously forms one of three shapes with an outstretched hand. These shapes are
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